Lycopene β-cyclase expression influences plant physiology, development and 
metabolism in tobacco plants

Kossler , Stella; Armarego-Marriott, T.; Tarkowska, Danuse; Tureckova, Veronika; Agrawal, S.; Mi, Jianing; Perez da Sousa, L.; Schöttler, M. A.; Schadach, A.; Fröhlich, A.; Bock, R.; Al-Babili, Salim; Ruf, S.; Sampathkumar, A.; Moreno, J.C.

doi:10.1093/jxb/erab029

Item

ITEM ACTIONSEXPORT

Add to Basket

Please note that a newer version of this item is available:
https://pure.mpg.de/pubman/item/item_3282002_8

DetailsSummary

Released

Journal Article

Lycopene β-cyclase expression influences plant physiology, development and metabolism in tobacco plants

MPS-Authors

/persons/resource/persons131117

Armarego-Marriott, T.
Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons202680

Agrawal, S.
Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons256352

Perez da Sousa, L.
Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons97392

Schöttler, M. A.
Photosynthesis Research, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons97372

Schadach, A.
Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons183349

Fröhlich, A.
Plant Cell Biology and Microscopy, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons97077

Bock, R.
Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons97364

Ruf, S.
Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons97369

Sampathkumar, A.
Plant Cell Biology and Microscopy, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

/persons/resource/persons192949

Moreno, J.C.
Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

External Resource

Link
(Any fulltext)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Kossler, S., Armarego-Marriott, T., Tarkowska, D., Tureckova, V., Agrawal, S., Mi, J., et al. (2021). Lycopene β-cyclase expression influences plant physiology, development and metabolism in tobacco plants. Journal of Experimental Botany, 72(7), 2544-2569. doi:10.1093/jxb/erab029.

Cite as: https://hdl.handle.net/21.11116/0000-0008-2FCB-0

Abstract

Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles in photoprotection and antioxidative processes. β-carotene is generated from lycopene by the lycopene β-cyclase (LCYB). Previously, we demonstrated that the introduction of the Daucus carota (carrot) DcLCYB1 gene into tobacco (cultivar Xanthi) resulted in increased levels of abscisic acid (ABA) and especially gibberellins (GAs), resulting in increased plant yield. In order to understand this phenomenon prior exporting this genetic strategy to crops, we generated tobacco (cultivar Petit Havana) mutants that exhibited a wide range of LCYB expression. Transplastomic plants expressing DcLCYB1 at high levels showed a wild-type-like growth, even though their pigment content was increased, and their leaf GA content was reduced. RNAi NtLCYB lines showed different reductions in NtLCYB transcript abundance, correlating with reduced pigment content and plant variegation. Photosynthesis (leaf absorptance, Fv/Fm, and ETRII) and plant growth were impaired. Remarkably, drastic changes in phytohormone content also occurred in the RNAi lines. However, external application of phytohormones was not sufficient to rescue their phenotypes, suggesting that altered photosynthetic efficiency might be another important factor explaining their reduced biomass. These results show that LCYB expression influences plant biomass by different mechanisms and suggests thresholds for LCYB expression levels that might be beneficial/detrimental for plant growth.