Laser Targeted Oligo Ligation (LTOL) to Identify DNA Sequences in the Vicinity 
of a Single Subnuclear Structure in a Single Cell

Anchel, David; Ching, Reagen

doi:org/10.1007/978-1-0716-0763-3_2,

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Laser Targeted Oligo Ligation (LTOL) to Identify DNA Sequences in the Vicinity of a Single Subnuclear Structure in a Single Cell

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Ching, Reagen
Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Citation

Anchel, D., & Ching, R. (2020). Laser Targeted Oligo Ligation (LTOL) to Identify DNA Sequences in the Vicinity of a Single Subnuclear Structure in a Single Cell. Methods in Molecular Biology, 2175, 11-21. doi:org/10.1007/978-1-0716-0763-3_2,.

Cite as: https://hdl.handle.net/21.11116/0000-0007-DF48-E

Abstract

Gene loci are organized around nuclear substructures, forming gene hubs which provide a level of transcriptional control. To date, most techniques used to investigate the genes in these hubs have been based on using material from bulk cells. This makes identifying specific gene associations difficult. Here we describe the Laser Targeted Oligo Ligation (LTOL) technique that was developed to identify DNA sequences around a single subnuclear structure on a single-cell basis by targeting these regions with two-photon irradiation.