Help Privacy Policy Disclaimer
  Advanced SearchBrowse




Journal Article

Proteome Turnover in the Spotlight: Approaches, Applications, and Perspectives


Langer,  Julian David       
Proteomics and Mass Spectrometry, Max Planck Institute of Biophysics, Max Planck Society;
Proteomics (Scientific Service Group), Max Planck Institute for Brain Research, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available

Ross, A. B., Langer, J. D., & Jovanovic, M. (2021). Proteome Turnover in the Spotlight: Approaches, Applications, and Perspectives. Molecular and Cellular Proteomics, 20: 100016. doi:10.1074/mcp.R120.002190.

Cite as: https://hdl.handle.net/21.11116/0000-0007-E8F8-C
In all cells, proteins are continuously synthesized and degraded to maintain protein homeostasis and modify gene expression levels in response to stimuli. Collectively, the processes of protein synthesis and degradation are referred to as protein turnover. At a steady state, protein turnover is constant to maintain protein homeostasis, but in dynamic responses, proteins change their rates of synthesis and degradation to adjust their proteomes to internal or external stimuli. Thus, probing the kinetics and dynamics of protein turnover lends insight into how cells regulate essential processes such as growth, differentiation, and stress response. Here, we outline historical and current approaches to measuring the kinetics of protein turnover on a proteome-wide scale in both steady-state and dynamic systems, with an emphasis on metabolic tracing using stable isotope–labeled amino acids. We highlight important considerations for designing proteome turnover experiments, key biological findings regarding the conserved principles of proteome turnover regulation, and future perspectives for both technological and biological investigation.