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Journal Article

Single channel recordings from basolateral and apical membranes of renal proximal tubules

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Gögelein,  Heinz
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Greger,  Rainer
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Gögelein, H., & Greger, R. (1984). Single channel recordings from basolateral and apical membranes of renal proximal tubules. Pflügers Archiv: European Journal of Physiology, 401, 424-426. doi:10.1007/BF00584348.


Cite as: http://hdl.handle.net/21.11116/0000-0007-EB2C-0
Abstract
A new method is described, which enables the recording of single ionic channels from the basolateral as well as the luminal membrane of renal proximal tubules with the patch-clamp technique. Segments of late proximal tubules of rabbit kidney are cannulated and perfused from one end. The other end is open and freely accessible to a patch pipette. The patch electrode can be moved against lateral cell membranes or can be inserted through the open end into the lumen and brought to contact with the brush-border membrane. Both, in the basolateral as well as in the luminal membrane, giga seals can be achieved. In both membranes, K+ selective channels could be identified.