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Activity-dependent spatially localized miRNA maturation in neuronal dendrites

MPG-Autoren

Sambandan,  S.
Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society;

Akbalik,  G.
Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society;

Kochen,  L.
Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society;

Glock,  C.
Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society;

Tushev,  G.
Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society;

Alvarez-Castelao,  B.
Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society;

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Schuman,  Erin M.
Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society;

Externe Ressourcen

https://www.ncbi.nlm.nih.gov/pubmed/28183980
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Zitation

Sambandan, S., Akbalik, G., Kochen, L., Rinne, J., Kahlstatt, J., Glock, C., et al. (2017). Activity-dependent spatially localized miRNA maturation in neuronal dendrites. Science, 355(6325), 634-637. doi:10.1126/science.aaf8995.


Zitierlink: https://hdl.handle.net/21.11116/0000-0007-EF03-9
Zusammenfassung
MicroRNAs (miRNAs) regulate gene expression by binding to target messenger RNAs (mRNAs) and preventing their translation. In general, the number of potential mRNA targets in a cell is much greater than the miRNA copy number, complicating high-fidelity miRNA-target interactions. We developed an inducible fluorescent probe to explore whether the maturation of a miRNA could be regulated in space and time in neurons. A precursor miRNA (pre-miRNA) probe exhibited an activity-dependent increase in fluorescence, suggesting the stimulation of miRNA maturation. Single-synapse stimulation resulted in a local maturation of miRNA that was associated with a spatially restricted reduction in the protein synthesis of a target mRNA. Thus, the spatially and temporally regulated maturation of pre-miRNAs can be used to increase the precision and robustness of miRNA-mediated translational repression.