Design of photocaged puromycin for nascent polypeptide release and 
spatiotemporal monitoring of translation

Buhr, F.; Kohl-Landgraf, J.; tom Dieck, S.; Hanus, C.; Chatterjee, D.; Hegelein, A.; Schuman, Erin M.; Wachtveitl, J.; Schwalbe, H.

doi:10.1002/anie.201410940

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Design of photocaged puromycin for nascent polypeptide release and spatiotemporal monitoring of translation

MPS-Authors

/persons/resource/persons208206

Schuman, Erin M.
Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society;

External Resource

https://www.ncbi.nlm.nih.gov/pubmed/25656536
(Any fulltext)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Buhr, F., Kohl-Landgraf, J., tom Dieck, S., Hanus, C., Chatterjee, D., Hegelein, A., et al. (2015). Design of photocaged puromycin for nascent polypeptide release and spatiotemporal monitoring of translation. Angew Chem Int Ed Engl, 54(12), 3717-21. doi:10.1002/anie.201410940.

Cite as: https://hdl.handle.net/21.11116/0000-0007-EF17-3

Abstract

The antibiotic puromycin, which inhibits protein translation, is used in a broad range of biochemical applications. The synthesis, characterization, and biological applications of NVOC-puromycin, a photocaged derivative that is activated by UV illumination, are presented. The caged compound had no effect either on prokaryotic or eukaryotic translation or on the viability of HEK 293 cells. Furthermore, no significant release of ribosome-bound polypeptide chains was detected in vitro. Upon illumination, cytotoxic activity, in vitro translation inhibition, and polypeptide release triggered by the uncaging of NVOC-puromycin were equivalent to those of the commercial compound. The quantum yield of photolysis was determined to be 1.1+/-0.2% and the NVOC-puromycin was applied to the detection of newly translated proteins with remarkable spatiotemporal resolution by using two-photon laser excitation, puromycin immunohistochemistry, and imaging in rat hippocampal neurons.