Cell-selective metabolic labeling of proteins

Ngo, J. T.; Champion, J. A.; Mahdavi, A.; Tanrikulu, I. C.; Beatty, K. E.; Connor, R. E.; Yoo, T. H.; Dieterich, D. C.; Schuman, Erin M.; Tirrell, D. A.

doi:10.1038/nchembio.200

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Journal Article

Cell-selective metabolic labeling of proteins

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Schuman, Erin M.
Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society;

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https://www.ncbi.nlm.nih.gov/pubmed/19668194
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Citation

Ngo, J. T., Champion, J. A., Mahdavi, A., Tanrikulu, I. C., Beatty, K. E., Connor, R. E., et al. (2009). Cell-selective metabolic labeling of proteins. Nat Chem Biol, 5(10), 715-7. doi:10.1038/nchembio.200.

Cite as: http://hdl.handle.net/21.11116/0000-0007-EF30-6

Abstract

Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected.