Functional expression of bacteriorhodopsin in oocytes allows direct measurement 
of voltage dependence of light induced H+ pumping

Nagel, Georg; Möckel, Bettina; Büldt, Georg; Bamberg, Ernst

doi:10.1016/0014-5793(95)01356-3

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Functional expression of bacteriorhodopsin in oocytes allows direct measurement of voltage dependence of light induced H⁺ pumping

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Nagel, Georg
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Bamberg, Ernst
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Nagel, G., Möckel, B., Büldt, G., & Bamberg, E. (1995). Functional expression of bacteriorhodopsin in oocytes allows direct measurement of voltage dependence of light induced H⁺ pumping. FEBS Letters, 377(2), 263-266. doi:10.1016/0014-5793(95)01356-3.

Cite as: https://hdl.handle.net/21.11116/0000-0008-0EF7-3

Abstract

We report on the first successful expression of the light driven H^+- pump, bacteriorhodopsin, into the plasma membrane of oocytes from Xenopus laevis. The light induced photocurrents which reflect the pumping of H^+- by BR were analysed under voltage clamp conditions. At least 100 active BR molecules per μm² were expressed in the plasma membrane so that both the voltage clamp and giant patch clamp method could be applied. We show that H^+- pumping by BR is modulated by the membrane potential, i.e. the pump current shows strong voltage dependence in the range measured between −165 mV to +60 mV.