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Journal Article

A simple method to reconstruct firing rates from dendritic calcium signals


Laurent,  Gilles
Neural systems Department, Max Planck Institute for Brain Research, Max Planck Society;

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Moreaux, L., & Laurent, G. (2009). A simple method to reconstruct firing rates from dendritic calcium signals. Front Neurosci, 2(2), 176-85. doi:10.3389/neuro.01.032.2008.

Cite as: http://hdl.handle.net/21.11116/0000-0008-07B0-9
Calcium imaging using fluorescent reporters is the most widely used optical approach to investigate activity in intact neuronal circuits with single-cell resolution. Calcium signals, however, are often difficult to interpret, especially if the desired output quantity is membrane voltage or instantaneous firing rates. Combining dendritic intracellular electrophysiology and multi-photon calcium imaging in vivo, we recently investigated the relationship between optical signals recorded with the fluorescent calcium indicator Oregon Green BAPTA-1 (OGB-1) and spike output in principal neurons in the locust antennal lobe. We derived from these experiments a simple, empirical and easily adaptable method requiring minimal calibration to reconstruct firing rates from calcium signals with good accuracy and 50-ms temporal resolution.