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Dynamic laser light scattering to determine size distributions of vesicles

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Ruf,  Horst
Molecular Biophysics Group, Max Planck Institute of Biophysics, Max Planck Society;

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Grell,  Ernst
Molecular Biophysics Group, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Ruf, H., Georgalis, Y., & Grell, E. (1989). Dynamic laser light scattering to determine size distributions of vesicles. In S. Fleischer, & B. Fleischer (Eds.), Methods in Enzymology (pp. 364-390). San Diego, California 92101, USA: Academic Press. doi:10.1016/s0076-6879(89)72024-3.


Cite as: http://hdl.handle.net/21.11116/0000-0008-00BF-1
Abstract
Publisher Summary: This chapter describes dynamic laser light scattering to determine size distributions of vesicles and presents a survey of the underlying principles with regard to vesicle suspensions. From a size distribution, it can be decided whether vesicles are homogeneous in size. Its characteristics can be used for controlling the stability of a sample and for studying processes such as aggregation and fusion. The nonmicroscopic methods requiring rather homogeneous populations with a well-defined shape are most appropriately applied to small unilamellar vesicles. Electron microscopy is used for vesicles having an extended size range. Because of the remarkable sensitivity of parameters to the size distribution of vesicles based on the composition of a medium, it is important to characterize and analyze each vesicle preparation.