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Journal Article

Caged-Ca2+: a new agent allowing liberation of free Ca2+ in biological systems by photolysis

MPS-Authors
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Grell,  Ernst
Molecular Biophysics Group, Max Planck Institute of Biophysics, Max Planck Society;

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Lewitzki,  Erwin
Molecular Biophysics Group, Max Planck Institute of Biophysics, Max Planck Society;

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Ruf,  Horst
Molecular Biophysics Group, Max Planck Institute of Biophysics, Max Planck Society;

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Bamberg,  Ernst
Transport Proteins Group, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Grell, E., Lewitzki, E., Ruf, H., Bamberg, E., Ellis-Davies, G., Kaplan, J. H., et al. (1989). Caged-Ca2+: a new agent allowing liberation of free Ca2+ in biological systems by photolysis. Cellular and Molecular Biology, 35(5), 515-522.


Cite as: https://hdl.handle.net/21.11116/0000-0008-0202-3
Abstract
The alkaline earth cation complexes of DM-nitrophen [1-(2-nitro-4,5-dimethoxyphenyl)-N,N,N',N'-tetrakis-[(oxycarbonyl) methyl]- 1,2-ethanediamine)] release the bound cation in the millisecond time range upon irradiation by a short UV-light pulse. This technique allows to generate cation (eg. Ca2+) concentration jumps or pulses in solution or in cellular systems. The physico-chemical properties of DM-nitrophen and its Mg2+, Ca2+ and Ba2+ complexes are investigated by employing spectrophotometric and potentiometric techniques. In case of Ca2+ a stability constant of the complex up to nearly 10(11) M-1 is found. The magnitude of representative Mg2+ and Ca2+ concentration jumps which can be generated under realistic experimental conditions are calculated on the basis of the thermodynamic parameters reported here.