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Conference Paper

Use of dynamic light scattering for the determination of size distributions of chylomicrons from human lymph


Ruf,  Horst
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Ruf, H., & Gould, B. J. (1997). Use of dynamic light scattering for the determination of size distributions of chylomicrons from human lymph. In R. C. Leif, A. V. Priezzhev, & T. Asakura (Eds.), Proceedings SPIE 2982 Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology. SPIE, International society for optics and photonics.

Cite as: https://hdl.handle.net/21.11116/0000-0008-0183-2
A sample of chylomicrons from human lymph was characterized using dynamic light scattering measurements. The stability of the sample was checked by carrying out series measurements at 3 different days, and was found to be very high during this period. The size distribution was determined by means of an extended version of the algorithm CONTIN that allowed normalization errors to be taken into account. We applied here a special evaluation scheme, where the data were corrected for these errors before final analysis. In addition to the intensity weighted size distribution obtained from this method we derived the size distributions of number and mass, utilizing the scattering amplitude functions of Mie. For these calculations the intensity-weighted size distribution was refined using an interpolation scheme to reduce problems associated with the use of mean values of the scattering amplitude functions for the intervals of the original size grid. The results showed that the chylomicron sample contained two classes of particles with intensity-weighted mean diameters of 107 nm and 320 nm. Number and mass distributions derived for the two major peaks indicated that practically all particles were small ones, but also that the few remaining large ones were carrying about 10 percent of the total mass.