The application of a potential-sensitive cyanine dye to rat small intestinal 
brush border membrane vesicles

Stiegert, Bruno; Burckhardt, Gerhard; Murer, Heini

doi:10.1016/0005-2736(83)90221-3

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The application of a potential-sensitive cyanine dye to rat small intestinal brush border membrane vesicles

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Burckhardt, Gerhard
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Stiegert, B., Burckhardt, G., & Murer, H. (1983). The application of a potential-sensitive cyanine dye to rat small intestinal brush border membrane vesicles. Biochimica et Biophysica Acta-Biomembranes, 732(1), 324-326. doi:10.1016/0005-2736(83)90221-3.

Cite as: https://hdl.handle.net/21.11116/0000-0008-05D1-6

Abstract

The sensitivity of the fluorescent dye, 3,3'-diethylthiadicarbocyanine (DiS-C₂(5], was too low for the detection of membrane potential changes in rat small intestinal membrane vesicles. Only after adding LaCl₃ or after fractionation of the intestinal membranes by free-flow electrophoresis could the dye be used to monitor electrogenic Na⁺-dependent transport systems. It is concluded that the response of this potential-sensitive dye is influenced by the negative surface charge density of the vesicles.