A cyanine dye as indicator of membrane electrical potential differences in 
brush border membrane vesicles. Studies with K+ gradients and Na+/amino acid 
cotransport

Burckhardt, Gerhard; Murer, Heini

doi:10.1016/B978-0-08-026824-8.50056-4

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A cyanine dye as indicator of membrane electrical potential differences in brush border membrane vesicles. Studies with K⁺ gradients and Na⁺/amino acid cotransport

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Burckhardt, Gerhard
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Burckhardt, G., & Murer, H. (1981). A cyanine dye as indicator of membrane electrical potential differences in brush border membrane vesicles. Studies with K⁺ gradients and Na⁺/amino acid cotransport. Oxford, New York: Pergamon Press. doi:10.1016/B978-0-08-026824-8.50056-4.

Cite as: https://hdl.handle.net/21.11116/0000-0008-2617-4

Abstract

Publisher Summary: Cyanine dyes are used to monitor membrane electrical potential differences in cells, organelles, and vesicles by optical methods. These potential-sensitive dyes differ with respect to molecular structure, charge, and permeability through membranes. Depending on the dye, potential-dependent binding to membranes and formation of dimers and higher aggregates are involved. This chapter describes the test of 3, 3’-diethylthiadicarbocyanine, a positively charged permeable cyanine dye as a membrane potential probe in brush border membrane vesicles from rat renal proximal tubules. The method is validated by investigating the response of the dye to imposition of various K⁺ gradients and thus different K⁺ diffusion potentials. The method is also applied to Na⁺-dependent transport of neutral amino acids expected to be potential-dependent. The chapter describes that the cyanine dye used is suggested as a powerful tool to study transport–dependent changes in membrane potential of isolated vesicles.