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Introduction to electrophysiology and epithelial transport: the use of fast concentration step experiments in the electrical analysis of tubular transport

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Frömter,  Eberhard
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Frömter, E. (1981). Introduction to electrophysiology and epithelial transport: the use of fast concentration step experiments in the electrical analysis of tubular transport. Oxford, New York: Pergamon Press. doi:10.1016/B978-0-08-026824-8.50050-3.


Cite as: https://hdl.handle.net/21.11116/0000-0008-2613-8
Abstract
Publisher Summary: This chapter discusses that the classical electrophysiological technique is a powerful tool in the hands of renal physiologists. The chapter describes the specific advantages of electrophysiological approach. (1) It directly determines the membrane potential, which is one of the conjugate driving forces for ion flow. (2) It can pass current across the membrane, which is not possible in vesicle preparations. (3) One can use ion selective electrodes to measure ion activities and to follow the time course of ion concentration changes in response to changes of system parameters from which net ion fluxes can be calculated. It also explains that electrophysiological techniques offer a unique tool, the microelectrode, which enables to monitor transport events of single cells under their normal living conditions and in their normal environment.