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Measurement of membrane transport in single cells by fluorenscence microphotolysis

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Peters,  Reiner
Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Peters, R. (1985). Measurement of membrane transport in single cells by fluorenscence microphotolysis. Trends in Biochemical Sciences: TIBS / International Union of Biochemistry and Molecular Biology, 10(6), 223-227-223-227. doi:10.1016/0968-0004(85)90133-1.


Cite as: https://hdl.handle.net/21.11116/0000-0008-0551-7
Abstract
Fluorescence microphotolysis (‘photobleaching’) has been widely used for studying the translational mobility of membrane proteins and lipids. Recently, the technique has been used for measuring, in single normal-sized cells, the transport of solutes through membranes. Such single-cell flux measurements could not be performed previously and open new possibilities to membrane transport research. This article discusses the methodological basis of fluorescence microphotolysis and two applications: anion transport in red cell ghosts and nucleocytoplasmic transport in cultured liver cells.