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Journal Article

Anion transport in single erythrocyte ghosts measured by fluorescence microphotolysis

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Peters,  Reiner
Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Passow,  Hermann
Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Peters, R., & Passow, H. (1984). Anion transport in single erythrocyte ghosts measured by fluorescence microphotolysis. Biochimica et Biophysica Acta-Biomembranes, 777(2), 334-338. doi:10.1016/0005-2736(84)90436-X.


Cite as: http://hdl.handle.net/21.11116/0000-0008-0556-2
Abstract
Fluorescence microphotolysis was used to measure in single resealed human erythrocyte ghosts the band 3-mediated transport of the fluorescent anion N-(7-nitrobenzofurazan-4-yl)-taurine (NBD-taurine). Transport was reduced to less than 5% of the control by the specific inhibitor 4,4′-diisothiocyanostilbene-2,2′-disulfonate (DIDS). The accuracy of the determination of the rate constant for NBD-taurine influx was approximately ± 15% as calculated from repetitive measurements in individual ghosts. The sample population distribution of the rate constant was slightly skewed towards values larger than the mean. The rate of NBD-taurine transport showed an optimum near pH 7. The Arrhenius plot was linear in the range from 28.5°C to 51°C with an apparent activation enthalphy of 21.4 kcal/mol.