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Nuclear localization of budgerigar fledgling disease virus capsid protein VP2 is conferred by residues 308-317

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Rihs,  Hans-Peter
Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society;
Institut für Mikro- und Molekularbiologie, Justus-Liebig-Universität Giessen, Germany;

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Peters,  Reiner
Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society;
Institut für Medizinische Physik, Westfälische Wilhelms-Universität Münster, 4400 Münster, Germany;

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Citation

Rihs, H.-P., Peters, R., & Hobom, G. (1991). Nuclear localization of budgerigar fledgling disease virus capsid protein VP2 is conferred by residues 308-317. FEBS Letters, 291(1), 6-8. doi:10.1016/0014-5793(91)81090-u.


Cite as: http://hdl.handle.net/21.11116/0000-0008-059B-4
Abstract
The capsid protein VP2 of budgerigar fledgling disease virus (BFDV) contains two sequences (residues 309-315 and 334-340) which are homologous to the prototypic nuclear localization sequence (NLS) of the simian virus 40 T-antigen. Using recombinant potential NLS-beta-galactosidase fusion proteins we identified amino acid residues 308-317 (VPKRKRKLPT) to be the NLS of BFDV capsid proteins VP2 and VP3. Microfluorometry studies show that the BFDV-VP2 signal is considerably more efficient in nuclear transport kinetics, than the NLS of SV40-VP2, corresponding to amino acid residues 317-326 (PNKKKRKLSR).