Unravelling the structural complexity of glycolipids with cryogenic infrared 
spectroscopy

Kirschbaum, Carla; Greis, Kim; Mucha, Eike; Kain, Lisa; Deng, Shenglou; Zappe, Andreas; Gewinner, Sandy; Schöllkopf, Wieland; Helden, Gert von; Meijer, Gerard; Savage, Paul B.; Marianski, Mateusz; Teyton, Luc; Pagel, Kevin

doi:10.1038/s41467-021-21480-1

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Unravelling the structural complexity of glycolipids with cryogenic infrared spectroscopy

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Kirschbaum, Carla
Institut für Chemie und Biochemie, Freie Universität Berlin;
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Greis, Kim
Institut für Chemie und Biochemie, Freie Universität Berlin;
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Mucha, Eike
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Gewinner, Sandy
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Schöllkopf, Wieland
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Helden, Gert von
Molecular Physics, Fritz Haber Institute, Max Planck Society;

/persons/resource/persons21859

Meijer, Gerard
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Pagel, Kevin
Institut für Chemie und Biochemie, Freie Universität Berlin;
Molecular Physics, Fritz Haber Institute, Max Planck Society;

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Citation

Kirschbaum, C., Greis, K., Mucha, E., Kain, L., Deng, S., Zappe, A., et al. (2021). Unravelling the structural complexity of glycolipids with cryogenic infrared spectroscopy. Nature Communications, 12: 1201. doi:10.1038/s41467-021-21480-1.

Cite as: https://hdl.handle.net/21.11116/0000-0008-0874-D

Abstract

Glycolipids are complex glycoconjugates composed of a glycan headgroup and a lipid moiety. Their modular biosynthesis creates a vast amount of diverse and often isomeric structures, which fulfill highly specific biological functions. To date, no gold-standard analytical technique can provide a comprehensive structural elucidation of complex glycolipids, and insufficient tools for isomer distinction can lead to wrong assignments. Herein we use cryogenic gas-phase infrared spectroscopy to systematically investigate different kinds of isomerism in immunologically relevant glycolipids. We show that all structural features, including isomeric glycan headgroups, anomeric configurations and different lipid moieties, can be unambiguously resolved by diagnostic spectroscopic fingerprints in a narrow spectral range. The results allow for the characterization of isomeric glycolipid mixtures and biological applications.