A crystallization screen based on alternative polymeric precipitants

Grimm, C.; Chari, A.; Reuter, K.; Fischer, U.

doi:10.1107/S0907444910009005

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsStatisticsRelease HistoryDetailsSummary

Released

Journal Article

A crystallization screen based on alternative polymeric precipitants

MPS-Authors

/persons/resource/persons133053

Chari, A.
Research Group of Structural Biochemistry and Mechanisms, MPI for Biophysical Chemistry, Max Planck Society;

External Resource

No external resources are shared

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Grimm, C., Chari, A., Reuter, K., & Fischer, U. (2010). A crystallization screen based on alternative polymeric precipitants. Acta Crystallographica Section D, 66(6), 685-697. doi:10.1107/S0907444910009005.

Cite as: http://hdl.handle.net/21.11116/0000-0008-0B9A-F

Abstract

Most commercially available crystallization screens are sparse-matrix screens with a predominance of inorganic salts and polyethylene glycols (PEGs) as precipitants. It was noted that commercially available screens are largely unsatisfactory for the purpose of the crystallization of multimeric protein and protein–nucleic acid complexes. This was reasoned to be a consequence of the redundancy in screening crystallization parameter space by the predominance of PEG as a precipitant in standard screens and it was suggested that this limitation could be overcome by introducing a variety of other organic polymers. Here, a set of 288 crystallization conditions was devised based on alternative polymeric precipitants and tested against a set of 20 different proteins/complexes; finally, a screen comprising the 96 most promising conditions designed to complement PEG- and salt-based commercial screens was proposed.