De novo protein structure determination from near-atomic-resolution cryo-EM maps

Ray Yu-Ruei, Ray Yu-Ruei; Kudryashev, Misha; Li, Xueming; Egelman, Edward H.; Basler, Marek; Cheng, Yifan; Baker, David; DiMaio, Frank

doi:10.1038/nmeth.3287

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De novo protein structure determination from near-atomic-resolution cryo-EM maps

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Citation

Ray Yu-Ruei, R.-Y.-R., Kudryashev, M., Li, X., Egelman, E. H., Basler, M., Cheng, Y., et al. (2015). De novo protein structure determination from near-atomic-resolution cryo-EM maps. Nature Methods, 12, 335-338. doi:10.1038/nmeth.3287.

Cite as: https://hdl.handle.net/21.11116/0000-0008-0FC6-9

Abstract

We present a de novo model-building approach that combines predicted backbone conformations with side-chain fit to density to accurately assign sequence into density maps. This method yielded accurate models for six of nine experimental maps at 3.3- to 4.8-Å resolution and produced a nearly complete model for an unsolved map containing a 660-residue heterodimeric protein. This method should enable rapid and reliable protein structure determination from near-atomic-resolution cryo-electron microscopy (cryo-EM) maps