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An inverse relationship between receptor internalization and the fraction of laterally mobile receptors for the vasopressin renal-type V2-receptor. An active role for receptor immobilization in down-regulation?

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Jans,  David A.
Emeritusgroup Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Peters,  Reiner
Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Fahrenholz,  Falk
Emeritusgroup Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Jans, D. A., Peters, R., & Fahrenholz, F. (1990). An inverse relationship between receptor internalization and the fraction of laterally mobile receptors for the vasopressin renal-type V2-receptor. An active role for receptor immobilization in down-regulation? FEBS Letters, 274(1-2), 223-226. doi:10.1016/0014-5793(90)81368-x.


Cite as: https://hdl.handle.net/21.11116/0000-0008-1FF5-2
Abstract
Lateral mobility of the vasopressin renal-type V2-receptor was investigated in LLC-PK1 porcine epithelial cells using the technique of fluorescence microphotolysis (photobleaching) and a rhodamine-labelled vasopressin analogue. At various times after ligand addition, cells were analyzed for both receptor lateral mobility and ligand internalization. The V2-receptor mobile fraction diminished from 0.9 to 0.43 over 60 min at 37°C, whereas the apparent lateral diffusion coefficient remained essentially unchanged (2-3 X 10-10 cm2s). Interestingly, the fraction of immobile V2-receptors corresponded exactly with the fraction of internalized receptors, implying a functional relationship. These observations together with comparable results reported for other polypeptide hormone receptors indicate a possible mechanistic role for receptor immobilization in the desensitization of hormonal response.