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A simple and fast method for the isolation of basolateral plasma membranes from rat small-intestinal epithelial cells

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Scalera,  Vito
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons258192

Storelli,  Carlo
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons258194

Storelli-Joss,  Christina
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons137691

Haase,  Winfried
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons257975

Murer,  Heini
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Scalera, V., Storelli, C., Storelli-Joss, C., Haase, W., & Murer, H. (1980). A simple and fast method for the isolation of basolateral plasma membranes from rat small-intestinal epithelial cells. Biochemical Journal, 186(1), 177-181. doi:10.1042/bj1860177.


Cite as: https://hdl.handle.net/21.11116/0000-0008-3100-0
Abstract
A method was developed for the analytical and preparative isolation of basolateral plasma membranes from rat small intestine. They were separated on a self-orientating Percoll (modified colloidal silica) gradient starting with a heavy microsomal-membrane fraction and involving centrifugation at 48,000 g for 1 h. (Na++ K+)-stimulated ATPase activity, used as a marker enzyme for the basolateral plasma membrane, is enriched 20-fold compared with that found in the homogenate of isolated intestinal epithelial cells.