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A simple isolation method for basal-lateral plasma membranes from rat kidney cortex

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Scalera,  Vito
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Huang,  Yu-Kuo
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Hildmann,  Bruno
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Scalera, V., Huang, Y.-K., Hildmann, B., & Murer, H. (1981). A simple isolation method for basal-lateral plasma membranes from rat kidney cortex. Membrane Biochemistry, 4(1), 49-61. doi:10.3109/09687688109065422.


Cite as: http://hdl.handle.net/21.11116/0000-0008-5103-9
Abstract
Basal-lateral membranes were separated in a self-orienting Percoll (modified colloidal silica) gradient from a heavy microsomal membrane fraction by centrifugation at 48,000g for 0.5 h. The (Na+-K+)-ATPase activity as a marker enzyme for the basal-lateral plasma membrane was 20-fold enriched by this procedure. The adenylate-cyclase activity measured in the basal-lateral membrane fraction was stimulated 6-fold by parathyrin and only up to 1.5-fold by arginine-vasopressin, calcitonin, or isoproterenol. The yield of basal-lateral plasma membranes was 5 to 10 percent of the amount initially present in the homogenate. The method is also applicable to the pig kidney.