Inhibition of anion transport across red blood cells with 1,2-cyclohexanedione

Zaki, Laila

doi:10.1016/0006-291x(81)91738-1

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Inhibition of anion transport across red blood cells with 1,2-cyclohexanedione

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Zaki, Laila
Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Zaki, L. (1981). Inhibition of anion transport across red blood cells with 1,2-cyclohexanedione. Biochemical and Biophysical Research Communications, 99(1), 243-251. doi:10.1016/0006-291x(81)91738-1.

Cite as: https://hdl.handle.net/21.11116/0000-0008-26E8-8

Abstract

The effects of three different α dicarbonyl reagents on SO₄ equilibrium exchange across the red cell membrane have been studied. It was found that 1,2-cyclohexanedione, an arginine specific reagent [1], causes inhibition of transport and a reduction of the capacity of the band 3 protein to bind the specific transport inhibitor H₂DIDS. The effect on the H₂DIDS binding capacity was, however, considerably smaller than the inhibition of sulfate transport indicating that the inhibition involved a modification of sites other than the H₂DIDS binding site. Squaric acid and 1,3-cyclohexanedione produced no inhibition under conditions where 1,2-cyclohexanedione was fully inhibitory. The results suggest that besides the lysine residues [2] involved in H₂DIDS-binding guanidino groups may also play a role in anion transport across the red blood cell membrane.