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The surface membrane of the small intestinal epithelial cell. I. Localization of adenyl cyclase

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/persons/resource/persons257975

Murer,  Heini
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;
Laboratorium für Biochemie, Federal Institute of Technology, Zurich Switzerland;
Department of Anatomy, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 U.S.A.;
Developmental Biology Center, Case Western Reserve University, Cleveland, Ohio 44106 U.S.A.;

/persons/resource/persons263137

Ammann,  Elvira
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;
Laboratorium für Biochemie, Federal Institute of Technology, Zurich Switzerland;
Department of Anatomy, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 U.S.A.;
Developmental Biology Center, Case Western Reserve University, Cleveland, Ohio 44106 U.S.A.;

/persons/resource/persons263139

Biber,  Jörg
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;
Laboratorium für Biochemie, Federal Institute of Technology, Zurich Switzerland;
Department of Anatomy, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 U.S.A.;
Developmental Biology Center, Case Western Reserve University, Cleveland, Ohio 44106 U.S.A.;

/persons/resource/persons260910

Hopfer,  Ulrich
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;
Laboratorium für Biochemie, Federal Institute of Technology, Zurich Switzerland;
Department of Anatomy, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106 U.S.A.;
Developmental Biology Center, Case Western Reserve University, Cleveland, Ohio 44106 U.S.A.;

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Citation

Murer, H., Ammann, E., Biber, J., & Hopfer, U. (1976). The surface membrane of the small intestinal epithelial cell. I. Localization of adenyl cyclase. Biochimica et Biophysica Acta-Biomembranes, 433(3), 509-519. doi:10.1016/0005-2736(76)90277-7.


Cite as: https://hdl.handle.net/21.11116/0000-0008-3EB4-8
Abstract
The subcellular distribution of adenyl cyclase was investigated in small intestinal epithelial cells. Enterocytes were isolated, disrupted and the resulting membranes fractionated by differential and sucrose gradient centrifugation. Separation of luminal (brush border) and contra-luminal (basolateral) plasma membrane was achieved on a discontinuous sucrose gradient.
The activity of adenyl cyclase was followed during fractionation in relation to other enzymes, notably those considered as markers for luminal and contraluminal plasma membrane. The luminal membrane was identified by the membrane-bound enzymes sucrase and alkaline phosphatase and the basolateral region by (Na+ + K+)-ATPase. Enrichment of the former two enzymes in purified luminal plasma membrane was 8-fold over cells and that of (Na+ + K+)-ATPase in purified basolateral plasma membranes was 13-fold. F- -activated adenyl cyclase co-purified with (Na+ + K+)-ATPase, suggesting a common localization on the plasma membrane. The distribution of K+ -stimulated phosphatase and 5′-nucleotidase also followed (Na+ + K+)-ATPase during fractionation.