Stable expression of gp80 (TRPM-2, clusterin), a secretory protein implicated 
in programmed cell death, in transfected BHK-21 cells

Pilarsky, Christian; Haase, Winfried; Koch-Brandt, Claudia

doi:10.1016/0167-4889(93)90086-5

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Stable expression of gp80 (TRPM-2, clusterin), a secretory protein implicated in programmed cell death, in transfected BHK-21 cells

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Haase, Winfried
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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引用

Pilarsky, C., Haase, W., & Koch-Brandt, C. (1993). Stable expression of gp80 (TRPM-2, clusterin), a secretory protein implicated in programmed cell death, in transfected BHK-21 cells. Biochimica et Biophysica Acta-Molecular Cell Research, 1179(3), 306-310. doi:10.1016/0167-4889(93)90086-5.

引用: https://hdl.handle.net/21.11116/0000-0008-28CC-6

要旨

The gp80 (TRPM-2, clusterin) cDNA cloned into an eucaryotic expression vector, was transfected into BHK-21 cells and stably transformed cell clones were obtained. Analysis of the gp80 glycoprotein complex produced in these cells demonstrated that the complex was glycosylated, proteolytically processed and secreted in a way similar to the gp80 glycoprotein complex expressed from the endogenous gene in MDCK cells. The analysis of the viability of the cells, the morphology and the state of the DNA in the transfected cells was unchanged when compared with the untransformed cells, demonstrating that the expression of the protein failed to elicit any signs of apoptosis in this system.