English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Renal V2 vasopressin receptor proteins: identification and enrichment

MPS-Authors
/persons/resource/persons206265

Fahrenholz,  Falk
Emeritusgroup Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons252926

Kojro,  Elzbieta
Emeritusgroup Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons258541

Plage,  G.
Emeritusgroup Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons258543

Müller,  M.
Emeritusgroup Physical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Fahrenholz, F., Kojro, E., Plage, G., & Müller, M. (1988). Renal V2 vasopressin receptor proteins: identification and enrichment. Journal of receptor research, 8(1-4), 283-294. doi:10.3109/10799898809048993.


Cite as: https://hdl.handle.net/21.11116/0000-0009-1591-B
Abstract
The synthesis of the tritium labelled photoreactive analogue of 1-deamino-vasopressin [1-(3-mercaptopropionic acid, 8-(N6-4-azido-phenylamidino)-lysine] vasopressin is described. This analogue retains a high affinity for hepatic V1 and renal V2 vasopressin receptors (apparent dissociation constant KD approximately 1-2 nM). A membrane protein from bovine kidney and pig kidney with an apparent relative molecular mass (Mr) of 30,000 was preferentially labelled and with lower yield a protein band with a Mr-value of 50,000 to 60,000. The photolabelled 30,000-Mr protein from bovine kidney was enriched by size-exclusion chromatography and by reversed-phase-high-performance liquid chromatography.