Serial femtosecond and serial synchrotron crystallography can yield data of 
equivalent quality: A systematic comparison

Mehrabi, P.; Bücker, R.; Bourenkov, G.; Ginn, H.; von Stetten, D.; Mueller-Werkmeister, H. M.; Kuo, A.; Morizumi, T.; Eger, B.; Ou, W.-L.; Oghbaey, S.; Sarracini, A.; Besaw, J.; Paré-Labrosse, O.; Meier, S.; Schikora, H.; Tellkamp, F.; Marx, A.; Sherrell, D. A.; Axford, D.; Owen, R. L.; Ernst, O. P.; Pai, Emil F.; Schulz, E.-C.; Miller, R. J. D.

doi:10.1126/sciadv.abf1380

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Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison

MPS-Authors

Mehrabi, P.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Department of Medical Biophysics, University of Toronto;
The Campbell Family Cancer Research Institute, Ontario, Cancer Institute;
Center for Free-Electron Laser Science;

Bücker, R.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Center for Free-Electron Laser Science;
Centre for Structural Systems Biology, Department of Chemistry, University of Hamburg;

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Paré-Labrosse, O.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Center for Free-Electron Laser Science;
Departments of Chemistry and Physics, University of Toronto;
International Max Planck Research School for Ultrafast Imaging & Structural Dynamics (IMPRS-UFAST), Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

Schikora, H.
Center for Free-Electron Laser Science;
Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

Tellkamp, F.
Center for Free-Electron Laser Science;
Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

Marx, A.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Center for Free-Electron Laser Science;

Schulz, E.-C.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Center for Free-Electron Laser Science;

Miller, R. J. D.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Center for Free-Electron Laser Science;
Departments of Chemistry and Physics, University of Toronto;
Department of Physics, Universität Hamburg;

External Resource

https://dx.doi.org/10.1101/2020.08.21.257170
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https://dx.doi.org/10.1126/sciadv.abf1380
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Citation

Mehrabi, P., Bücker, R., Bourenkov, G., Ginn, H., von Stetten, D., Mueller-Werkmeister, H. M., et al. (2021). Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison. Science Advances, 7(12): eabf1380. doi:10.1126/sciadv.abf1380.

Cite as: https://hdl.handle.net/21.11116/0000-0008-2F57-3

Abstract

For the two proteins myoglobin and fluoroacetate dehalogenase, we present a systematic comparison of crystallographic diffraction data collected by serial femtosecond (SFX) and serial synchrotron crystallography (SSX). To maximize comparability, we used the same batch of micron-sized crystals, the same sample delivery device, and the same data analysis software. Overall figures of merit indicate that the data of both radiation sources are of equivalent quality. For both proteins, reasonable data statistics can be obtained with approximately 5000 room-temperature diffraction images irrespective of the radiation source. The direct comparability of SSX and SFX data indicates that the quality of diffraction data obtained from these samples is linked to the properties of the crystals rather than to the radiation source. Therefore, for other systems with similar properties, time-resolved experiments can be conducted at the radiation source that best matches the desired time resolution.