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Functional characterization of proton antiport regulation in the thylakoid membrane

MPS-Authors
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Uflewski,  M.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Mielke,  S.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Correa Galvis,  V.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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von Bismarck,  T.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Chen,  X.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Tietz,  E.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Ruß,  J.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Luzarowski,  M.
Small Molecules, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Sokolowska,  E.
Small Molecules, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Skirycz,  A.
Small-Molecule Signalling, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Schöttler,  M. A.
Photosynthesis Research, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Armbruster,  U.
Regulation of Photosynthesis, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Citation

Uflewski, M., Mielke, S., Correa Galvis, V., von Bismarck, T., Chen, X., Tietz, E., et al. (2021). Functional characterization of proton antiport regulation in the thylakoid membrane. Plant Physiology. doi:10.1093/plphys/kiab135.


Cite as: https://hdl.handle.net/21.11116/0000-0008-2FB6-7
Abstract
VC generated transgenic plants. XC, ET, JR, SM and MU performed western blot analyses. UA and SM performed co-immunoprecipitations. MU generated cTP-YFP fusions and localized fusions using confocal microscopy. SM performed BN-PAGE analysis. JE and IF performed MS analysis on BN slices. MU produced recombinant protein in E. coli and performed size exclusion chromatography together with ML and AS. ES and AS performed MS analysis of recombinant protein. SM, MU and ET performed Chl a fluorescence measurements during light fluctuations. MAS measured 77K Chl a fluorescence emission spectra, ECS kinetics and Cytf redox state. MAS and UA performed Chl a fluorescence and P700 light response curves and steady state measurements, respectively. TvB carried out simultaneous CO2 assimilation and Chl a fluorescence measurements. UA wrote manuscript with help from all authors.