Indications of several nucleotide binding sites on the Na+K+-ATPase molecule

Koepsell, Hermann

doi:10.1016/0020-711X(80)90088-9

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Indications of several nucleotide binding sites on the Na⁺K⁺-ATPase molecule

MPS-Authors

/persons/resource/persons253157

Koepsell, Hermann
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Koepsell, H. (1980). Indications of several nucleotide binding sites on the Na⁺K⁺-ATPase molecule. International Journal of Biochemistry, 12(1-2), 301-305. doi:10.1016/0020-711X(80)90088-9.

Cite as: https://hdl.handle.net/21.11116/0000-0008-32BE-A

Abstract

1. The effect of different ATP concentrations on the inhibition of purified membrane-bound Na⁺-K⁺-ATPase by different inhibitors was investigated.
2. Trypsin, the SH-group reagent 5,5'dithiobis(2-nitrobenzoic acid) and the covalently binding ATP analogue 6-((3-carboxy-4-nitrophenyl)thiol)-9-β-d-ribofuranosyl-purine 5'triphosphate were employed as inhibitors.
3. The effects of ATP on enzyme inhibition were observed in three concentration ranges different from those previously employed to determine the high affinity ATP binding.
4. The data suggest that four types of nucleotide binding sites are present on the Na⁺-K⁺-ATPase molecule.