English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Torasemide inhibits NaCl reabsorption in the thick ascending limb of the loop of Henle

MPS-Authors
/persons/resource/persons257508

Wittner,  Monika
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons258882

di Stefano,  Antonio
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons256831

Schlatter,  Eberhard
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

/persons/resource/persons255425

Greger,  Rainer
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

External Resource
No external resources are shared
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Wittner, M., di Stefano, A., Schlatter, E., Delarge, J., & Greger, R. (1986). Torasemide inhibits NaCl reabsorption in the thick ascending limb of the loop of Henle. Pflügers Archiv: European Journal of Physiology, 407(6), 611-614. doi:10.1007/BF00582640.


Cite as: http://hdl.handle.net/21.11116/0000-0008-3EBD-F
Abstract
Torasemide (1-isopropyl-(4-(3-methylphenylamino)pyrid-3-yl)urea) is a new diuretic. The present study examines the effects of this substance in the isolated perfused thick ascending limb (TAL) of mouse and rabbit kidney. In cortical TAL segments of the rabbit, torasemide added to the lumen perfusate led to a fall in equivalent short circuit current ( = transepithelial voltage divided by transepithelial resistance, which corresponds to the rate of chloride reabsorption) with a half maximal inhibition concentration of 3 X 10-7 mol/l. This effect was accompanied by a hyperpolarization of the luminal and basolateral membrane from -78 to -81 mV and from -72 to -81 mV, respectively. A similar hyperpolarization of both membrane voltages was also observed in medullary TAL segments of the mouse. Torasemide, added to the basolateral perfusate of cortical TAL segments of the rabbit, also inhibited the equivalent short circuit current. However, 3 X 10-5 mol/l were necessary for a half maximal inhibition. The fall in the equivalent short circuit current was accompanied by a significant increase in transepithelial resistance from 34 to 38 omega cm2, by an increase in the fractional resistance of the basolateral membrane, and by a hyperpolarization mainly of the basolateral membrane. Again, similar results were obtained in the medullary TAL segment of the mouse. The strong inhibitory effect of torasemide from the lumen side can be explained by an interference with the Na+ 2Cl-K+ carrier in the luminal membrane. In fact, torasemide apparently is structurally related to furosemide