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Journal Article

Cl- -channels in the apical cell membrane of the rectal gland "induced" by cAMP

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Greger,  Rainer
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Schlatter,  Eberhard
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Gögelein,  Heinz
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Greger, R., Schlatter, E., & Gögelein, H. (1985). Cl- -channels in the apical cell membrane of the rectal gland "induced" by cAMP. Pflügers Archiv: European Journal of Physiology, 403, 446-448. doi:10.1007/BF00589260.


Cite as: https://hdl.handle.net/21.11116/0000-0008-3ED6-2
Abstract
Isolated rectal gland tubules (n≈1000) of dogfish (Squalus Acanthias) were perfused in vitro. Individual channels in the apical and basolateral cell membrane were recorded with the patch clamp method. K+-channels were present in excised membrane patches of the basolateral membrane in stimulated (dbcAMP + forskolin + adenosine) and in nonstimulated state. Cl−-channels were found only in patches of the apical cell membrane when the tubule was stimulated. Cell attached recordings and simultaneous transepithelial PD measurements were obtained while the segment was stimulated. It is shown that concomitant with the increase in lumen negative PD “silent” membrane patches of the apical cell membrane suddenly develop Cl−-channel activity. It is concluded that stimulation of rectal gland tubules “activates” Cl−-channels in the apical cell membrane.