Unmasking of a potassium leak in resealed human red blood cell ghosts

Wood, Phillip G.; Rossleben, U.

doi:10.1016/0005-2736(79)90235-9

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Unmasking of a potassium leak in resealed human red blood cell ghosts

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Wood, Phillip G.
Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Rossleben, U.
Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Wood, P. G., & Rossleben, U. (1979). Unmasking of a potassium leak in resealed human red blood cell ghosts. Biochimica et Biophysica Acta-Biomembranes, 553(2), 320-325. doi:10.1016/0005-2736(79)90235-9.

Cite as: https://hdl.handle.net/21.11116/0000-0008-40CE-8

Abstract

A selective potassium leak is observed in resealed, human red blood cell ghosts when hemolysis is performed with distilled water at pH 6.5, 0° C. The leak, which has a maximum near pH 6.7, is suppressed when either magnesium or a chelating agent is present in the hemolysing medium. The potassium leak has the additional property that it can be suppressed after resealing by washing the ghost membranes in a medium containing a low concentration of ATP or EDTA. The data suggest that through the dilution of endogenous chelating agents at hemolysis a potassium leak may be unmasked.