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Is hexokinase present in the basal lateral membranes of rat kidney proximal tubular epithelial cells?

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Keljo,  David J.
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;
Department of Physiology, University of Pennsylvania, Philadelphia, Pa. 19104 U.S.A.;

/persons/resource/persons259932

Kleinzeller,  Arnost
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;
Department of Physiology, University of Pennsylvania, Philadelphia, Pa. 19104 U.S.A.;

/persons/resource/persons257975

Murer,  Heini
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;
Department of Physiology, University of Pennsylvania, Philadelphia, Pa. 19104 U.S.A.;

/persons/resource/persons257977

Kinne,  Rolf
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;
Department of Physiology, University of Pennsylvania, Philadelphia, Pa. 19104 U.S.A.;

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Citation

Keljo, D. J., Kleinzeller, A., Murer, H., & Kinne, R. (1978). Is hexokinase present in the basal lateral membranes of rat kidney proximal tubular epithelial cells? Biochimica et Biophysica Acta-Biomembranes, 508(3), 500-512. doi:10.1016/0005-2736(78)90095-0.


Cite as: http://hdl.handle.net/21.11116/0000-0008-49C2-B
Abstract
The possible presence of hexokinase in basal lateral membranes from rat kidney proximal tubules was investigated. Basal lateral membranes were obtained from homogenates of rat kidney cortex by differential centrifugation and free flow electrophoresis. They were further purified by density gradient centrifugation. Hexokinase activity was measured as the phosphorylation of D-[U14C]glucose. Throughout the purification of the membranes, the specific activity of hexokinase decreased while that of (Na+ + K+)-ATPase increased. Hexokinase activity in all fractions could be quantitatively accounted for in terms of cytosolic and mitochondrial enzyme contributions. It is concluded that there is no hexokinase activity in basal lateral membranes from rat kidney.