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Isolated membrane vesicles in the evaluation of the nature, localization, and regulation of renal transport processes

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Kinne,  Rolf
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Kinne, R., & Schwartz, I. (1978). Isolated membrane vesicles in the evaluation of the nature, localization, and regulation of renal transport processes. Kidney International, 14(6), 547-556. doi:10.1038/ki.1978.163.


Cite as: http://hdl.handle.net/21.11116/0000-0008-49CB-2
Abstract
The isolation of membranes from defined segments of the nephron and defined regions of renal epithelial cells has provided a new approach to the analysis of renal tubular transport phenomena and hormone action. The new approach makes it possible: (a) to distinguish between the role of the plasma membrane and the role of other cellular components in transcellular transport and hormonal regulation; (b) to localize the transport elements situated in the two opposite poles of the cell, namely, the basal-lateral and the luminal membranes; and (c) to characterize these transport elements in terms of driving forces, molecular properties, and sensitivity to regulatory influences.