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Journal Article

Does Dimeric Melittin Occur in Aqueous Solutions?

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Schubert,  Dieter
Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Pappert,  Gunter
Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Boss,  Karin
Department of Cell Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Schubert, D., Pappert, G., & Boss, K. (1985). Does Dimeric Melittin Occur in Aqueous Solutions? Biophysical Journal, 48(2), 327-329. doi:10.1016/S0006-3495(85)83786-3.


Cite as: https://hdl.handle.net/21.11116/0000-0008-4AA6-A
Abstract
Melittin, a peptide from bee venom, is known to undergo a monomer / tetramer conversion in aqueous solutions. We have studied the possible participation of dimers in the association equilibrium of melittin by sedimentation equilibrium experiments in the analytical ultracentrifuge and subsequent mathematical analysis of the concentration distributions obtained. It was found that the dimeric state is not significantly populated, the contribution of dimer to the total peptide weight probably being below 0.5%.