Cholesterol Slows down the Lateral Mobility of an Oxidized Phospholipid in a 
Supported Lipid Bilayer

Plochberger, Birgit; Stockner, Thomas; Chiantia, Salvatore; Brameshuber, Mario; Weghuber, Julian; Hermetter, Albin; Schwille, Petra; Schuetz, Gerhard J.

doi:10.1021/la1026202

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Cholesterol Slows down the Lateral Mobility of an Oxidized Phospholipid in a Supported Lipid Bilayer

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Plochberger, B., Stockner, T., Chiantia, S., Brameshuber, M., Weghuber, J., Hermetter, A., et al. (2010). Cholesterol Slows down the Lateral Mobility of an Oxidized Phospholipid in a Supported Lipid Bilayer. Langmuir, 26(22), 17322-17329. doi:10.1021/la1026202.

Cite as: https://hdl.handle.net/21.11116/0000-0008-4C0C-7

Abstract

We investigated the mobility and phase-partitioning of the fluorescent oxidized phospholipid analogue 1-palmitoyl-2-glutaroyl-sn-glycero-3-phospho-N-Alexa647-ethanolamine (PGPE-Alexa647) in supported lipid bilayers. Compared to the conventional phospholipid dihexadecanoylphosphoethanolamine (DHPE)-Bodipy we found consistently higher diffusion constants. The effect become dramatic when immobile obstacles were inserted into the bilayer. which essentially blocked the diffusion of DHPE-Bodipy but hardly influenced the movements of PGPE-Alexa647. In a supported lipid bilayer made of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), the differences in probe mobility leveled off with increasing cholesterol content. Using coarse-grained molecular dynamics simulations, we could ascribe this effect to increased interactions between the oxidized phospholipid and the membrane matrix, concomitant with a translation in the headgroup position of the oxidized phospholipid: at zero cholesterol content, its headgroup is shifted to the outside of the DOPC headgroup region, whereas increasing cholesterol concentrations pulls the headgroup into the bilayer plane.