Unbiased proteomic profiling of host cell extracellular vesicle composition and 
dynamics upon HIV-1 infection

Martin-Jaular, Lorena; Nevo, Nathalie; Schessner, Julia P.; Tkach, Mercedes; Jouve, Mabel; Dingli, Florent; Loew, Damarys; Witwer, Kenneth W.; Ostrowski, Matias; Borner, Georg H. H.; Thery, Clotilde

doi:10.15252/embj.2020105492

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection

MPS-Authors

/persons/resource/persons229376

Schessner, Julia P.
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons136451

Borner, Georg H. H.
Borner, Georg / Systems Biology of Membrane Trafficking, Max Planck Institute of Biochemistry, Max Planck Society;

External Resource

https://www.embopress.org/doi/full/10.15252/embj.2020105492#support-information-section
(付録資料)

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

embj.2020105492.pdf
(出版社版), 3MB

付随資料 (公開)

There is no public supplementary material available

引用

Martin-Jaular, L., Nevo, N., Schessner, J. P., Tkach, M., Jouve, M., Dingli, F., Loew, D., Witwer, K. W., Ostrowski, M., Borner, G. H. H., & Thery, C. (2021). Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection. EMBO Journal, 40:. doi:10.15252/embj.2020105492.

引用: https://hdl.handle.net/21.11116/0000-0009-74E1-6

要旨

Cells release diverse types of extracellular vesicles (EVs), which transfer complex signals to surrounding cells. Specific markers to distinguish different EVs (e.g. exosomes, ectosomes, enveloped viruses like HIV) are still lacking. We have developed a proteomic profiling approach for characterizing EV subtype composition and applied it to human Jurkat T cells. We generated an interactive database to define groups of proteins with similar profiles, suggesting release in similar EVs. Biochemical validation confirmed the presence of preferred partners of commonly used exosome markers in EVs: CD81/ADAM10/ITGB1, and CD63/syntenin. We then compared EVs from control and HIV-1-infected cells. HIV infection altered EV profiles of several cellular proteins, including MOV10 and SPN, which became incorporated into HIV virions, and SERINC3, which was re-routed to non-viral EVs in a Nef-dependent manner. Furthermore, we found that SERINC3 controls the surface composition of EVs. Our workflow provides an unbiased approach for identifying candidate markers and potential regulators of EV subtypes. It can be widely applied to in vitro experimental systems for investigating physiological or pathological modifications of EV release.