Morphologische und biochemische Untersuchungen über die Oberflächenstruktur der 
Bürstensaummembran der Rattenniere /Surface structure of Isolated brushborder 
of rat kidney: Morphological and biochemical analysis

Pockrandt-Hemstedt, Hannelore; Schmitz, Jürgen Erik; Kinne-Saffran, Evamaria; Kinne, Rolf

doi:10.1007/BF00586210

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Morphologische und biochemische Untersuchungen über die Oberflächenstruktur der Bürstensaummembran der Rattenniere /Surface structure of Isolated brushborder of rat kidney: Morphological and biochemical analysis

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Pockrandt-Hemstedt, Hannelore
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Schmitz, Jürgen Erik
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Kinne-Saffran, Evamaria
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Kinne, Rolf
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Pockrandt-Hemstedt, H., Schmitz, J. E., Kinne-Saffran, E., & Kinne, R. (1972). Morphologische und biochemische Untersuchungen über die Oberflächenstruktur der Bürstensaummembran der Rattenniere /Surface structure of Isolated brushborder of rat kidney: Morphological and biochemical analysis. Pflügers Archiv: European Journal of Physiology, 333, 297-313. doi:10.1007/BF00586210.

Cite as: https://hdl.handle.net/21.11116/0000-0008-7235-C

Abstract

The action of trypsin (E.C. 3.4.4.4) and papain (E.C. 3.4.4.10) on the surface structure of the microvillus membrane was investigated using isolated brushborder fragments of rat kidney cortex. Negative staining was employed to demonstrate the surface structure. The composition of the membrane was determined by biochemical analysis.

Untreated membranes show a smooth surface without any structural arrangement. These membranes contain alkaline phosphatase (E.C. 3.1.3.1), aminopeptidase (E.C. 3.4.1.2), protein and 12 μg hexosamine per mg protein.

Treatment with trypsin lowers the equilibrium density of the brushborder fragments from 1.17 to 1.15 and releases glycoproteins from the membranes which contain 54% of the hexosamine present in the starting material. There is no change in the content of alkaline phosphatase and aminopeptidase following trypsin treatment. Negative staining now reveals the presence of particles with a diameter of 40 Å on the surface of the membrane. When brushborder fragments are treated with papain the surface particles as well as glycoproteins are removed. Aminopeptidase activity is no longer present in the brushborder membrane and the equilibrium density is decreased to 1.12.

It is proposed that the composition of the surface of kidney microvilli is as follows: particles with aminopeptidase activity are attached to a membrane matrix which contains alkaline phosphatase, other proteins and hexosamine. Both structure are covered by a glycoprotein layer which gives the microvillus surface its smooth appearance.