Datensatz

Zusammenfassung

Effect of parathyroid hormone and cyclic adenosine 3′, 5′-monophosphate on isotonic fluid reabsorption : Polarity of proximal tubular cells. Isotonic fluid reabsorption (J_v) of rat renal proximal tubules was examined by the shrinking droplet method in combination with simultaneous perfusion of blood capillaries. Sensitivity of J_v measurement was improved by using each punctured tubule for control measurements: 1) Parathyroid hormone (PTH) on the contraluminal cell side reduced J_v in a dose-response behavior. The maximal inhibition was achieved at a PTH concentration of 10^-6 M, the half maximal inhibition at a concentration of 3 × 10^-9 M. PTH on the luminal cell side had a small inhibitory effect. 2) Cyclic AMP inhibited J_v preferentially when applied to the luminal cell side. On the luminal cell side, both cyclic AMP and dibutyryl cyclic AMP inhibited J_v in a similar dose-dependent behavior. Concentrations of both nucleotides as low as 10^-10 M had a definite inhibitory effect. Tested at a high concentration, N⁶-butyryl cyclic AMP was almost as effective as cyclic AMP. Deoxy cyclic AMP, 5′ AMP, cyclic guanosine monophosphate (cyclic GMP), dibutyryl cyclic GMP had no effect. ATP inhibited J_v to a very small extent. 3) The reduction of J_v after administration of PTH and dibutyryl cyclic AMP was not additive. The similar inhibitory effect of PTH at the contraluminal cell face and of cyclic AMP at the luminal cell face suggests the following sequence of events in the mediation of the action of PTH: 1) activation of adenylate cyclase by PTH in the contraluminal cell membrane, and 2) action of the generated cyclic AMP on the luminal cell membrane. The interaction of cyclic AMP and the luminal cell membrane is initiated at the luminal cell surface.