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Journal Article

CRISPR-Cas in Streptococcus pyogenes


Le Rhun,  Anaïs
Max Planck Unit for the Science of Pathogens, Max Planck Society;

Charpentier,  Emmanuelle
Max Planck Unit for the Science of Pathogens, Max Planck Society;

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Le Rhun, A., Escalera-Maurer, A., Bratovič, M., & Charpentier, E. (2019). CRISPR-Cas in Streptococcus pyogenes. RNA biology, 16(4), 380-389. doi:10.1080/15476286.2019.1582974.

Cite as: http://hdl.handle.net/21.11116/0000-0008-744B-2
The discovery and characterization of the prokaryotic CRISPR-Cas immune system has led to a revolution in genome editing and engineering technologies. Despite the fact that most applications emerged after the discovery of the type II-A CRISPR-Cas9 system of Streptococcus pyogenes, its biological importance in this organism has received little attention. Here, we provide a comprehensive overview of the current knowledge about CRISPR-Cas systems from S. pyogenes. We discuss how the interplay between CRISPR-mediated immunity and horizontal gene transfer might have modeled the evolution of this pathogen. We review the current literature about the CRISPR-Cas systems present in S. pyogenes (types I-C and II-A), and describe their distinctive biochemical and functional features. Finally, we summarize the main biotechnological applications that have arisen from the discovery of the CRISPR-Cas9 system in S. pyogenes.