The minimum requirements for the maintenance of active sodium transport across 
the isolated salivary duct epithelium of the rabbit

Knauf, Heinrich

doi:10.1007/BF00586212

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The minimum requirements for the maintenance of active sodium transport across the isolated salivary duct epithelium of the rabbit

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Knauf, Heinrich
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;
Medizinische Universitätsklinik, D-7800 Freiburg im Breisgau, Germany;

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Citation

Knauf, H. (1972). The minimum requirements for the maintenance of active sodium transport across the isolated salivary duct epithelium of the rabbit. Pflügers Archiv: European Journal of Physiology, 333, 326-336. doi:10.1007/BF00586212.

Cite as: https://hdl.handle.net/21.11116/0000-0008-B881-6

Abstract

The main excretory duct of the rabbit submaxillary gland was isolated and studied in a lucite perfusion chamber. Measurements of transepithelial electrical potential difference (p.d.) and specific electrical wall resistance (R_m) were performed and short-circuit-current (SCC) was determined under conditions of different test solutions placed at the blood side of the duct. 1. High molecular compounds such as albumin or dextran are required in the bathing medium to maintain transport function. 2. Active transport of sodium from the lumen to the interstitium can be maintained only when there is both pyruvate and acetate present in a bicarbonate Ringer's bathing medium. Isocitrate can substitute for pyruvate and acetate. SCC was found to be 1.7±0.2 mA/cm² with the artificial bathing fluid as compared to 1.9±0.2 mA/cm² with rabbit serum placed at the blood side of the duct. 3. In the absence of bicarbonate Na⁺ transport is reduced to 55±10% of control. 4. Potassium in a 4 mM concentration on the blood side proved to be indispensable for transport function. In the absence of interstitial potassium the Na⁺ conductance of the luminal cell membrane is reduced. Rubidium and cesium ions can partially substitute for potassium.