Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
Zur Ablage hinzufügen
  Lokale TagsFreigabegeschichteDetailsÜbersicht

Freigegeben
Zeitschriftenartikel

Distribution of parathyroid hormone-stimulated adenylate cyclase in plasma membranes of cells of the kidney cortex

MPG-Autoren
/persons/resource/persons260904

Shlatz,  Linda J.
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;
Department of Physiology and Biophysics, Mount Sinai Medical and Graduate Schools of the City University of New York, 10029, New York, N.Y.; USA;

/persons/resource/persons260906

Schwartz,  Irving L.
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;
Department of Physiology and Biophysics, Mount Sinai Medical and Graduate Schools of the City University of New York, 10029, New York, N.Y.; USA;

/persons/resource/persons257981

Kinne-Saffran,  Evamaria
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;
Department of Physiology and Biophysics, Mount Sinai Medical and Graduate Schools of the City University of New York, 10029, New York, N.Y.; USA;

/persons/resource/persons257977

Kinne,  Rolf
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;
Department of Physiology and Biophysics, Mount Sinai Medical and Graduate Schools of the City University of New York, 10029, New York, N.Y.; USA;

Externe Ressourcen
Es sind keine externen Ressourcen hinterlegt
Volltexte (beschränkter Zugriff)
Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte in PuRe verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Shlatz, L. J., Schwartz, I. L., Kinne-Saffran, E., & Kinne, R. (1975). Distribution of parathyroid hormone-stimulated adenylate cyclase in plasma membranes of cells of the kidney cortex. Journal of Membrane Biology, 24(2), 131-144. doi:10.1007/BF01868619.


Zitierlink: https://hdl.handle.net/21.11116/0000-0008-BB2C-5
Zusammenfassung
Free flow electrophoresis was employed to separate renal cortical plasma membranes into luminal (brush border microvilli) and contraluminal (basal-lateral membrane) fractions. During the separation adenylate cyclase activity was found to parallel the activity of Na+-K+-activated ATPase, an enzyme which is present in contraluminal but not in luminal membranes. In the basal-lateral membrane fraction the specific activities of adenylate cyclase and Na+-K+-activated ATPase were 4.4 and 4.6 times greater, respectively, than in the brush border fraction. The adenylate cyclase of the basal-lateral membrane fraction was specifically stimulated by parathyroid hormone which maximally increased enzyme activity eightfold. The biologically active (1-34) peptide fragment of paratyhroid hormone produced a 350% increase in adenylate cyclase activity. In contrast, calcitonin, epinephrine and vasopressin maximally stimulated the enzyme by only 55, 35 and 30%, respectively. These results indicate that adenylate cyclase, specifically stimulated by parathyroid hormone, is distributed preferentially in the contraluminal region of the plasma membrane of renal cortical epithelial cells.