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Journal Article

Rapid multi-plane phase-contrast microscopy reveals torsional dynamics in flagellar motion

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Bae,  Albert J.
Laboratory for Fluid Physics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

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Gholami,  Azam
Laboratory for Fluid Physics, Pattern Formation and Biocomplexity, Max Planck Institute for Dynamics and Self-Organization, Max Planck Society;

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Citation

Mojiri, S., Isbaner, S., Mühle, S., Jang, H., Bae, A. J., Gregor, I., et al. (2021). Rapid multi-plane phase-contrast microscopy reveals torsional dynamics in flagellar motion. Biomedical Optics Express, 12: 6, pp. 3169. doi:10.1364/BOE.419099.


Cite as: https://hdl.handle.net/21.11116/0000-0008-9153-6
Abstract
High speed volumetric optical microscopy is an important tool for observing rapid processes in living cells or for real-time tracking of sub-cellular components. However, the 3D imaging capability often comes at the price of a high technical complexity of the imaging system and/or the requirement of demanding image analysis. Here, we propose a combination of conventional phase-contrast imaging with a customized multi-plane beam-splitter for enabling simultaneous acquisition of images in eight different focal planes. Our method is technically straightforward and does not require complex post-processing image analysis. We apply our multi-plane phase-contrast microscope to the real-time observation of the fast motion of reactivated Chlamydomonas axonemes with sub-µm spatial and 4 ms temporal resolution. Our system allows us to observe not only bending but also the three-dimensional torsional dynamics of these micro-swimmers.