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Fluorometrisch-enzymatische Mikro- und Ultramikrobestimmung von Inulin und Glucose / Fluorometric-Enzymatic determination of inulin and glucose in the micro and ultramicro range

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Zwiebel,  Ralf K.H.
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Höhmann,  Bernhard
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Frohnert,  Peter P.
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Baumann,  Karl
Department of Physiology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Zwiebel, R. K., Höhmann, B., Frohnert, P. P., & Baumann, K. (1969). Fluorometrisch-enzymatische Mikro- und Ultramikrobestimmung von Inulin und Glucose / Fluorometric-Enzymatic determination of inulin and glucose in the micro and ultramicro range. Pflügers Archiv: European Journal of Physiology, 307, 127-132. doi:10.1007/BF00586469.


Cite as: http://hdl.handle.net/21.11116/0000-0008-BB28-9
Abstract
Enzymatic methods of inulin and glucose determination are adapted to sample sizes in the micro-and ultramicro range and modified for fluorometric measurements. Using a highly sensitive ultramicro method inulin and/or glucose could be measured exactly in concentrations of 5 mgm per cent in samples of 1 nl (1×10−9 l) corresponding to absolute amounts of 1×10−12 gm.