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Serial Electron Diffraction Data Processing With diffractem and CrystFEL

MPG-Autoren
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Bücker,  R.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Center for Free-Electron Laser Science, Hamburg;
Centre for Structural Systems Biology, Department of Chemistry, University of Hamburg;

/persons/resource/persons202699

Hogan-Lamarre,  P.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;
Department of Physics, University of Toronto;
Department of Chemistry, University of Toronto;

Externe Ressourcen

https://dx.doi.org/10.3389/fmolb.2021.624264
(Verlagsversion)

https://arxiv.org/abs/2011.02977
(Preprint)

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Volltexte (frei zugänglich)

fmolb-08-624264.pdf
(Verlagsversion), 5MB

Ergänzendes Material (frei zugänglich)

5429703.zip
(Ergänzendes Material), 3MB

Zitation

Bücker, R., Hogan-Lamarre, P., & Miller, R. J. D. (2021). Serial Electron Diffraction Data Processing With diffractem and CrystFEL. Frontiers in Molecular Biosciences, 8: 624264. doi:10.3389/fmolb.2021.624264.


Zitierlink: https://hdl.handle.net/21.11116/0000-0008-935C-B
Zusammenfassung
Serial electron diffraction (SerialED) is an emerging technique, which applies the snapshot data-collection mode of serial X-ray crystallography to three-dimensional electron diffraction (3D Electron Diffraction), forgoing the conventional rotation method. Similarly to serial X-ray crystallography, this approach leads to almost complete absence of radiation damage effects even for the most sensitive samples, and allows for a high level of automation. However, SerialED also necessitates new techniques of data processing, which combine existing pipelines for rotation electron diffraction and serial X-ray crystallography with some more particular solutions for challenges arising in SerialED specifically. Here, we introduce our analysis pipeline for SerialED data, and its implementation using the CrystFEL and diffractem program packages. Detailed examples are provided in extensive supplementary code.