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Journal Article

Dynamic relocalization of cytosolic type III secretion system components prevents premature protein secretion at low external pH

MPS-Authors
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Wimmi,  Stephan
Research Group Bacterial Secretion Systems, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Balinovic,  Alexander
Microbial Networks, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Jeckel,  Hannah
Max Planck Research Group Bacterial Biofilms, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Selinger,  Lisa
Research Group Bacterial Secretion Systems, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Lampaki,  Dimitrios
Research Group Bacterial Secretion Systems, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

Eisemann,  Emma
Research Group Bacterial Secretion Systems, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254232

Drescher,  Knut
Max Planck Research Group Bacterial Biofilms, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Endesfelder,  Ulrike
Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Diepold,  Andreas
Research Group Bacterial Secretion Systems, Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

External Resource

https://doi.org/10.1038/s41467-021-21863-4
(Publisher version)

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Citation

Wimmi, S., Balinovic, A., Jeckel, H., Selinger, L., Lampaki, D., Eisemann, E., et al. (2021). Dynamic relocalization of cytosolic type III secretion system components prevents premature protein secretion at low external pH. Nature Communications, 12(1): 1625. doi:10.1038/s41467-021-21863-4.


Cite as: https://hdl.handle.net/21.11116/0000-0008-BDEA-C
Abstract
Many bacterial pathogens use a type III secretion system (T3SS) to
manipulate host cells. Protein secretion by the T3SS injectisome is
activated upon contact to any host cell, and it has been unclear how
premature secretion is prevented during infection. Here we report that
in the gastrointestinal pathogens Yersinia enterocolitica and Shigella
flexneri, cytosolic injectisome components are temporarily released from
the proximal interface of the injectisome at low external pH, preventing
protein secretion in acidic environments, such as the stomach. We show
that in Yersinia enterocolitica, low external pH is detected in the
periplasm and leads to a partial dissociation of the inner membrane
injectisome component SctD, which in turn causes the dissociation of the
cytosolic T3SS components. This effect is reversed upon restoration of
neutral pH, allowing a fast activation of the T3SS at the native target
regions within the host. These findings indicate that the cytosolic
components form an adaptive regulatory interface, which regulates T3SS
activity in response to environmental conditions. Many bacterial
pathogens use a type III secretion system (T3SS) to inject effector
proteins into host cells. Here, Wimmi et al. show that the external pH
regulates the assembly of T3SS cytosolic components in intestinal
pathogens, thus preventing T3SS activity in the stomach and allowing
T3SS reactivation in the intestine.