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Journal Article

Correction of Frameshift Mutations in the atpB Gene by Translational Recoding in Chloroplasts of Oenothera and Tobacco.

MPS-Authors
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Malinova,  Irina
Cytoplasmic and Evolutionary Genetics, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Zupok,  Arkadiusz
Cytoplasmic and Evolutionary Genetics, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Yaneva-Roder,  Liliya
Cytoplasmic and Evolutionary Genetics, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Szymanski,  Witold G.
Signalling Proteomics, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;
Core Facility Mass Spectrometry and Proteomics, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Ruf,  Stephanie
Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Bock,  Ralph
Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Greiner,  Stephan
Cytoplasmic and Evolutionary Genetics, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;

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Citation

Malinova, I., Zupok, A., Massouh, A., Aurel Schottler, M., Meyer, E. H., Yaneva-Roder, L., et al. (2021). Correction of Frameshift Mutations in the atpB Gene by Translational Recoding in Chloroplasts of Oenothera and Tobacco. The Plant cell. doi:10.1093/plcell/koab050.


Cite as: https://hdl.handle.net/21.11116/0000-0008-BDFC-8
Abstract
Translational recoding, also known as ribosomal frameshifting, is a
process that causes ribosome slippage along the messenger RNA, thereby
changing the amino acid sequence of the synthesized protein. Whether the
chloroplast employs recoding is unknown. I-iota, a plastome mutant of
Oenothera (evening primrose), carries a single adenine insertion in an
oligoA stretch [11A] of the atpB coding region (encoding a beta-subunit
of the ATP synthase). The mutation is expected to cause synthesis of a
truncated, non-functional protein. We report that a full-length AtpB
protein is detectable in I-iota leaves, suggesting operation of a
recoding mechanism. To characterize the phenomenon, we generated
transplastomic tobacco lines in which the atpB reading frame was altered
by insertions or deletions in the oligoA motif. We observed that
insertion of two adenines was more efficiently corrected than insertion
of a single adenine, or deletion of one or two adenines. We further show
that homopolymeric composition of the oligoA stretch is essential for
recoding, as an additional replacement of AAA lysine codon by AAG
resulted in an albino phenotype. Our work provides evidence for the
operation of translational recoding in chloroplasts. Recoding enables
correction of frameshift mutations and can restore photoautotrophic
growth in the presence of mutation that otherwise would be lethal.