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Journal Article

FRET Microscopy in Yeast

MPS-Authors
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Skruzny,  Michal
Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
Microbial Networks, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Pohl,  Emma
Department of Organismic Interactions, Alumni, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Abella Guerra,  Marc
Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
Microbial Networks, Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Citation

Skruzny, M., Pohl, E., & Abella Guerra, M. (2019). FRET Microscopy in Yeast. BIOSENSORS-BASEL, 9(4): 122. doi:10.3390/bios9040122.


Cite as: https://hdl.handle.net/21.11116/0000-0008-BED6-1
Abstract
Forster resonance energy transfer (FRET) microscopy is a powerful
fluorescence microscopy method to study the nanoscale organization of
multiprotein assemblies in vivo. Moreover, many biochemical and
biophysical processes can be followed by employing sophisticated FRET
biosensors directly in living cells. Here, we summarize existing FRET
experiments and biosensors applied in yeasts Saccharomyces cerevisiae
and Schizosaccharomyces pombe, two important models of fundamental
biomedical research and efficient platforms for analyses of bioactive
molecules. We aim to provide a practical guide on suitable FRET
techniques, fluorescent proteins, and experimental setups available for
successful FRET experiments in yeasts.